Part:BBa_K1364021:Design

Integrative plasmid for Bacillus subtilis (pSBbs4E)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 523
  Illegal XbaI site found at 2768
  Illegal SpeI site found at 2864
  Illegal PstI site found at 2623
  Illegal PstI site found at 4219
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 523
  Illegal EcoRI site found at 5739
  Illegal SpeI site found at 2
  Illegal SpeI site found at 2864
  Illegal PstI site found at 16
  Illegal PstI site found at 2623
  Illegal PstI site found at 4219
  Illegal NotI site found at 9
  Illegal NotI site found at 5745
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 523
  Illegal EcoRI site found at 5739
  Illegal BglII site found at 2751
  Illegal BamHI site found at 5723
  Illegal XhoI site found at 3118
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 5739
  Illegal suffix found at 2
  Illegal EcoRI site found at 523
  Illegal XbaI site found at 2768
  Illegal SpeI site found at 2864
  Illegal PstI site found at 2623
  Illegal PstI site found at 4219
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 5739
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 523
  Illegal XbaI site found at 2768
  Illegal XbaI site found at 5754
  Illegal SpeI site found at 2
  Illegal SpeI site found at 2864
  Illegal PstI site found at 16
  Illegal PstI site found at 2623
  Illegal PstI site found at 4219
  Illegal NgoMIV site found at 3082
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 4045
  

Source

bla